Bio Enzyme Lab

Enzyme Lab Experiments Problem How can we demonst site how enzymes consort? What happens if we alter the environment of an enzyme? Materials Glucose Test StripsTest TubesPipettesRaw HamburgLettucePotato Raw LiverChalkBeakersDairy milk sugar TabletWaterSugar only Cups Hot PlateKnifeGlovesSkim MilkGlow SticksPeroxide Hypothesis 1. If we reposition the environment via temperature the impertinence father go a representation Its intensity will change 2. If heat content peroxide is added to a certain food liver then It would bubble 3. If a lactaid enzyme to take out the It would sepa gait unconscious process (A) 1. Collect three novelty sticks. 2.Boil pissing in 400 mL beaker add 1st glowstick for 30 minutes. 3. protrude second glowstick in freezer for 30 minutes 4. Leave trinity glow stick at room temperature for 30 minutes 5. line up and record findings. Data Collected (A) steer a glow stick in all(prenominal) environment below. Using a scale 1-3 1 being normal intensity 3 being brigh visitation intensity BeakerObservation Freezer1 boiling Water3 Room Temperature (control)2 Procedure (B) 1. Collect sample of raw liver, potato, raw hamburg, lettuce and chalk. 2. Place each sample in individual establish subway system in rack. 3. Observe samples prior to adding peroxide. 4.Add 1 mL of peroxide to each analyse tube 5. Observe and record findings Data Collected (B) answer after hydrogen peroxide is added. Using a scale of 0-5 0 Being no bubbles 5 being the most bubbles Test tubeObservation ahead adding hydrogen peroxideObservation after adding hydrogen peroxide 1 liverDark brown in color (expired meat)5 2 potatoInner slice with some skin2 3 raw hamburgNormal in color4 4 lettuceFresh green lettuce1 5 chalk Yellow colored chalk0 Procedure (C) Preparation 1. Enzyme Solution Add maven lactase tablet to cc ml of water. posit until the tablet is dissolved. . Skim Milk This ups longing contains lactose. 3. sucrose SolutionAdd 5 grams of sugar to 100 ml of water. Stir until the sugar is dissolved. 4. Denatured enzyme Solution 1. Place 20 ml of enzyme event into a try on tube. 2. Add 200 ml of water to a 400 ml beaker. 3. Place the test tube in the beaker. educate sure it does non spill out. 4. Place the beaker and the test tube on a hot plate. 5. Boil the water for 30 minutes. 6. Let the root word cool to room temperature. Procedure (C-1) 1. Collect 6 mL of check out milk, place 2mL in each test tube (3 test tubes of scan milk). . Collect 4mL of sucrose solution, place 2mL in each test tube (2 test tubes total). 3. Observe and record initial observations. 4. Add 1mL of enzyme solution to a skim milk test tube add 1mL of water to second skim milk test tube, ad 1mL denatured enzyme solution to third skim milk test tube. 5. Add 1mL of enzyme solution to 1st test tube of sucrose solution add 1mL of water to 2nd test tube of sucrose solution. 6. Insert Glucose test strip in wch test tube (5 total). Wait 2 minutes. 7. Obser ve and record whether or not glucose is present and how much.Test tubeInitial ObservationGlucose Test strip Is glucose present 1. 2 ml of skim milk and 1ml of the enzyme solutionBubbles3000 Glucose 2. 2 ml of skim milk and 1 ml of waterNo bubbles 2 layers300 Glucose 3. 2 ml of skim milk and 1 ml of denatured solutionBubbles and layers 0 Glucose 4. 2 ml of sucrose solution and 1 ml of enzyme solutionClear liquidYellow in color zero glucose 5. 2 ml of sucrose solution and 1 ml of waterClear liquid Yellow in color no glucose What happens when the enzyme is denatured? The Enzyme does not work. Result Questions 1. In which beaker did the glow stick glow the most? wherefore do you think that is? The beaker that contained hot water. The heat from the water will speed up the chemical rate of reaction taking place, and therefore will glow brighter the cold one will pass on its rate of reaction slowed, and thus be dimmer. 2. What did the glow stick show or so enzymes and different environme nts? How does changing the temperature affect the rate of an enzyme-controlled experiment? The increasing temperature increases molecular motion and may increase the bet of times an enzyme contacts and combines with a substrate molecule.Temperature may also influence the incarnation of the enzyme molecule, making it fit better with the substrate. 3. What effect may change in PH have on an enzyme activity? The three-dimensional structure of a protein leaves certain side chains exposed. These side chains may realise ions from the environment. Under the right conditions, a group of positively charged hydrogen ions may accumulate on certain parts of an enzyme. A change in pH disrupts an enzymes shape and structure. When the pH changes an enzymes structure, the enzyme cant do its job. Changes in pH break the delicate bonds that maintain an enzymes shape.An enzyme will unravel, or denature, and become deceitful in a different pH. Stomach enzymes work in a super acidic environment of p H 2. A little way down the digestive tract, intestine enzymes need a pH of 8. 4. Why did the hamburger and liver react differently with hydrogen peroxide? They have natural catalase enzymes which were broken down with the presence of hydrogen peroxide. 5. What is the job of the lactase enzyme? Lactase is an enzyme which breaks down lactose, a sugar found in milk and other dairy products. 6. In which test tubes was the glucose present? Why?In test tube 1 the enzyme broke down the lactose . Lactose is a disaccharide of glucose and brain sugar. The lactose tablet contained lactase which hydrolyzed the lactose into constituent galactose and glucose. Glucose is naturally occurring in skim milk thus in test tube 2 found in a much littler amount than test tube 1. 7. What happened when the enzyme was denatured or altered by heat? Did it work the same? Was glucose present? There was no glucose present. It did not work at all. Summary What the class learn today about the jobs of enzymes? Wh at questions does the class still have?